Clonal Evaluation and Genetic Divergence Studies in Mulberry Genotypes

Manickavasagam Mithilasri *

Centre for Climate Change and Disaster Management (CCC&DM), Anna University, India.

K. T. Parthiban

Forest College and Research Institute, Mettupalayam, India.

R. Kalpana

Department of Sericulture, Forest College and Research Institute, Mettupalayam, India.

Shankar S.M.

Department of Physics, Don Bosco Arts and Science College, Dharamapuri, India.

*Author to whom correspondence should be addressed.


Aims: This study aims to evaluate the wood quality and leaf quality traits of selected mulberry clones and assess the genetic divergence among them, providing valuable insights for the development of superior genotypes with enhanced economic value for the sericulture and agroforestry sectors.

Study design: Twenty-one genetic resources of mulberry were collected from various regions in India and evaluated through clonal test in a Row Column Design to assess growth attributes and genetic divergence.

Place and Duration of Study: The study was conducted in India, and the systematic progeny test and clonal evaluation were carried out in Forest College and Research Institute, Mettupalayam during 2018-2022.

Methodology: The selected clones were evaluated for growth attributes such as plant height, diameter at breast height (DBH), volume, number of branches, leaf length, leaf width, petiole length, number of leaves, and leaf area. Genetic divergence was estimated using D2 statistics, and clustering of clones was performed using the 'GENRES' statistical package.

Results: The study identified several clones with significantly higher growth attributes, highlighting their potential for selection in breeding and cultivation programs. Variability and heritability studies indicated that volume exhibited the highest heritability, suggesting strong genetic control and potential for improvement.

Conclusion: Clones MI-0718, MI-0807, and MI-0845 showed superior growth performance and genetic divergence, indicating their suitability for further breeding and improvement programs. The study provides insights into the genetic variability of mulberry clones and emphasizes selecting superior clones for enhancing wood volume and overall productivity.

Keywords: Correlation, growth attributes, genetic diversity, mulberry

How to Cite

Mithilasri , M., Parthiban , K. T., Kalpana , R., & , S. S. (2024). Clonal Evaluation and Genetic Divergence Studies in Mulberry Genotypes. Asian Research Journal of Agriculture, 17(2), 147–159.


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Das S, Dutta Pand, Sengupta D. Genetic variability, heritability, and genetic advance for wood quality traits in mulberry (Morus spp.). International Journal of Chemical Studies. 2018;6(3), 2826-2830.

Kumar A, Singh RK, Singh AK. Assessment of genetic diversity for leaf quality traits in mulberry (Morus spp.) using RAPD markers. International Journal of Agriculture, Environment and Biotechnology, 2020;13(5):883-889.

Rajendran P. Genetic variability and association studies in half-sib progenies of Puvarasu (Thespesia populnea L. Sol. ex. Correa) in coastal regions of Thiruvarur District, Tamil Nadu. Electronic Journal of Plant Breeding. 2018;9(3):966-971.

Pitcher JA, Dorn DE. A new form for reporting hardwood superior tree candidates. Proc.5th central states forest tree improvement, Wooster, Ohil.1966; 7-12.

Panse VJ, Sukhatme. PV. Statistical methods for agricultural workers, New delhi: ICAR;1967.

Mahalanobis PC. Statistical study of the Chinese head; 1928.

Saini Pawan, SS Chauhan, Aftab A Shabnam, Lal Chand, and Narender Negi. Genetic variability and trait association analysis for agro-morphological markers in mulberry genetic resources from Kashmir, India." Int. J. Curr. Microbiol. App. Sci. 2018;7(4):1799-1812.

Sharma NK, Burman U, Tewari JC, Bhora MD, Harsh LN. Variability studies in pod and seed characteristics of Prosopis juliflora (SW.) DC. Indian Journal of Forest. 1994;17(2):161-165.

Suresh K, Jalaja SK, Banerjee R, Trivedy K. Genetic variability, correlation and path analysis in physiological and yield attributes in mulberry (Morus spp.). J. Crop Weed. 2017;13(1):28-33.

Parthiban, KT, Selvan, RT, Palanikumaran B, Krishnakumar,N. Variability and genetic diversity studies on Neolamarckia cadamba genetic resources. Journal of Tropical Forest Science. 2019;31(1):90-98.

Gawali AS, Wagh RS, Sonawane CJ. Genetic variability in growth and seed traits of Jatropha curcas L. germplasm for genetic tree improvement. Forest Res. 2016;5(171):2.

Sathyanarayana K, Sangannavar, PA. Correlation and Path Analysis of Morpho-physiological Parameters Contributing to Leaf Yield in Mulberry (Morus alba L.,) under Salt Affected Soils in India.Int. J. Curr. Microbiol. App. Sci. 2020;9(5):2118-2133.

Dhillon RS, Verma RC, Dhanda SK, Sheokand R, Kumari S. Genetic divergence based on quantitative variation for some seed traits in plus trees of neem (Azadirachta indica A. Juss). Indian Journal of Agroforestry. 2009;11 (1).

Murthy Keshava BC, Puttaraju HP, Shailaja Hittalmani. Genetic variability and correlation studies in selected mulberry (Morus spp.) germplasm accessions. Electronic Journal of Plant Breeding. 2010;1(3):351-355.

Doss SG, Rahman MS, Debnath S, Ghosh MK, Sau H, Ghosh PL, Sarkar A. Variability, heritability and genetic advance in nine germplasm lines of mulberry (Morus spp.). Indian Journal of Genetics. 2006;66:169-170.

Kanna SU, Krishnakumar N Jailani KM, Kather MD. Variability, heritability and genetic advance of Ailanthus excelsa. Int. J. Curr. Microbiol. App. Sci. 2019;8(7):1508-1517.

Mahendran S, Rajendran P, Parthiban KT, Sivakumar K, Prabakaran P. Genetic variability and association studies in half-sib progenies of Puvarasu (Thespesia populnea L. Sol. ex. Correa) in coastal regions of Thiruvarur District, Tamil Nadu. Electronic Journal of Plant Breeding. 2018;9 (3):966-971.

Choudhary Punit, Singh NB, Sharma JP, Archna Verma. Estimation of Genetic Parameters among Intra and Interspecific Progenies of Tree Willows. Indian Forester. 2016;142 (12):1157-1163.

Kaushik N, Chhabra AK. Genetic divergence analysis in Ailanthus excelsa based on morphological traits. Indian Journal of Agricultural Sciences. 2017;87(2):173-178.

Behera LK, Jha SK, Gunaga RP, Nayak D, Tandel MB, Jadeja DB. Genetic variability and correlation study for growth characters among clones of Eucalyptus. Int J Chem Stud. 2017;5 (6):763-765.

Shree MP, Krishnakumar N, Parthiban KT. Genetic Diversity and Relationships of Neolamarckia cadamba(Roxb.) Bosser progenies through cluster analysis. Tropical Plant Research. 2018;5(1):107-15.

Johnson Herbert W, Robinson HF, Comstock R Ev. Genotypic and phenotypic correlations in soybeans and their implications in selection. Agronomy journal. 1955a;47(10):477-483.

Divakara BN. Assessing variability and divergence of Jatropha curcas Linn. germplasm under ex-situ conditions. Forest Res. 2017;6(201):2.

Al- Aysh Firas, Hussein Kutma, Mahmood Serhan, Abdulla Al-Zoubai, Mohammad Abdelsalam Al-Naseer, DAG Village. Genetic analysis and correlation studies of yield and fruit quality traits in tomato (Solanum lycopersicum L.). New York Science Journal. 2012;5 (10):142-145.

Chikkalingaiah Y, Vijayashekar B Geetha Devi T, Sadatulla. Correlation and path co-efficient analysis in different accessions of mulberry.In: Proc. National Workshop on Seri-Biodiversity Conservation. 2009;7-8 March.

Dar Rukhsar Ahmad, Jag Paul Sharma, Gupta RK, Sandeep Chopra. Studies on correlation and path analysis for yield and physico chemical traits in tomato (Lycopersicon esculentum Mill). Vegetos. 2011;24 (2):136-141.

De Souza, Lívia M, Paulo César T Melo, Reginaldo R Luders, and Arlete MT Melo. Correlations between yield and fruit quality characteristics of fresh market tomatoes. Horticultura Brasileira. 2012;30:627-631.

Harer PN, Lad DB, Bhor TJ. Correlation and path analysis studies in tomato. Journal of Maharashtra Agricultural Universities (India). 2002;47(10):477-483.

Islam BM, Ivy NA, Rasul MG, Zakaria M. Character association and path analysis of exotic tomato (Solanum lycopersicum L.) genotypes. Bangladesh Journal of Plant Breeding and Genetics. 2010;23(1):13-8.

Sushmitha C, Murali S, Ahalya BN. Genetic variation for Vegetative and Reproductive Traits in Mulberry (Morus spp) Accessions; 2021.

Rao AA, Chauhan SS, Radhakrishnan R, Tikader A, Borpuzari MM, Kamble CK. Distribution, variation and conservation of mulberry (Morus spp.) genetic resources in the arid zone of Rajasthan, India. Bioremediation, Biodiversity and Bioavailability. 2011;5(1):52-62.

Meena Himanshu, Ashok Kumar, Rajni Sharma, Sanjeev Kumar Chauhan, Krishana Mohan Bhargava. Genetic variation for growth and yield parameters in half-sib progenies of Melia azedarach (Linn.). Turkish journal of Agriculture and Forestry. 2014;38 (4):531-539.

Sangram C, Keerthika A. Genetic variability and association studies among morphological traits of Leucaena leucocephala (Lam.) de Wit. genetic resources. 2013;5(1):526-536.

Pandey Deepak, Vasudha Pandey, Sanjay Tripathi, Salil K Tewari. Path analysis in Populus Deltoides Bart: 8 year age.Indian Forester. 1997;123(8):755-758.

Sundaram Reeja. Genetic variability and divergence studies in Prosopis juliflora DC. Journal of Pharmacognosy and Phytochemistry. 2018;7(4):3479-3484.

Pavithra Hosalli Ramaswamy, Balakrishna Gowda, Kuthi Thammaiah Prasanna, Manchanahally Byrappa Shivanna. Pod and seed traits in candidate plus trees of Pongamia pinnata (L.) Pierre from southern peninsular India in relation to provenance variation and genetic variability. Journal of Crop Science and Biotechnology. 2013;16 (2):131-142.

Chavan Raju, Viswanath S, Shivanna H. Correlation and path coefficient analysis in five Casuarina species for productivity of biomass. Karnataka Journal of Agricultural Sciences. 2012; 24 (5):156-187.

Ashok Kumar, Dhillon GPS. Clonal testing of Eucalyptus clones and estimation of their genetic parameters. Indian Forester. 2016;142 (2):127-132.

Priyanka GP, Biradar IB, Satish D, Sandhyarani Nishani, Nagesh Naik and Manukumar. H.R. Character association and path analysis studies in tamarind (Tamarindus indica L.). genotypes Journal of Pharmacognosy and Phytochemistry. 2021;10 (2):606-609.

Aslam Mohd, Zafar A Reshi, Siddiqi TO. Genetic Divergence In Half-Sib Progenies Of Pinus wallichiana A. B. Jackson Plus Trees In The Kashmir Himalaya, India. Tropical Ecology. 2011;52 (2):201-208.

Chauhan SK, Sehgal RN. Genetic divergence among progenies of Himalayan long leaf pine. Indian Journal of Forestry. 2001; 24:65-71.

Kala S, Kumaran K. Genetic divergence studies among the progenies of plus trees in Bixa orellana, L. Indian Forester. 2012;138 (9):828-834.

Chaturvedi OP, Pandey N. Genetic divergence in Bombax ceiba L. germplasms. Silvae Genetica. 2001;50(3-4):99-102.

Bagchi SK. Divergence in Tectona grandis. Ann. For. 2000;8(1):25-27.

Kumaran K, Nesamani K, Govinda Rao M. Correlation and path coefficient studies in simarouba glauca DC. Indian Forester. 2010;136 (3):322-330.

Panse VG, Sukhatme PV. Statistical methods for agricultural workers. Indian Council of Agricultural Research (ICAR) Publication, New Delhi, India; 1987.

Parthiban KT. Mini clonal technology for tree crops: Forestry technologies-a complete value chain approach; 2017.

Rahman MS, Islam SMS. Genetic diversity analysis based on morphological characters in mulberry (Morus spp.). Journal of Bio-Science. 2020b; 28:111-119.

Rao CR. Advanced statistical methods in biometric research; 1952.

Rao, GR, Shanker, AK, Srinivas,I, Korwar,GR and Venkateswarlu,B. Diversity and variability in seed characters and growth of Pongamia pinnata (L.) Pierre accessions. Trees. 2011;25 (4):725-734.

Sehgal RN, Chauhan SK, Dhall SP. Half-sib progeny evaluation in Chir pine. Silvae Genetica (Germany); 1995.

Selvan RT, Parthiban KT. Clonal evaluation and genetic divergence studies in Neolamarckia cadamba roxb. Electronic Journal of Plant Breeding. 2018; 9 (2):692-704.

Tasisa, Jiregna, Derbew Belew, and Kassahin Bantte. Genetic associations analysis among some traits of tomato (Lycopersicon esculentum Mill.) genotypes in West Showa, Ethiopia. International Journal of Plant Breeding and Genetics. 2012;6 (3):129-139.