Efficacy of Biopesticide Formula Containing Streptomyces sp. and Trichoderma sp. against Southern Green Stink Bug (Nezara viridula) on Soybean (Glycine max L.)
Asian Research Journal of Agriculture,
Page 218-226
DOI:
10.9734/arja/2022/v15i4372
Abstract
Streptomyces sp. and Trichoderma sp. are soil microorganisms isolated from shallot fields that can act as biological agents and increase crop production. Nezara viridula, the southern green stink bug, is the leading pest of soybean during the generative period, which can cause damage up to 80%. This study aimed to determine the efficacy of a liquid biopesticide formula using a mixture of coconut water and potato extract containing Streptomyces sp. and Trichoderma sp. This study used a randomized block design. The first factor was the time of application and the second factor was the concentration level. There were 8 treatment combinations and 2 controls. Each treatment combination was repeated three times. Probit LC50 and LT50 were performed to determine the effectiveness of biopesticides. The calculation of probit analysis obtained results of 84,443 ppm or about 84% for LC50, while the LT50 analysis obtained results of 4.7 days.
Keywords:
- Lethal concentration
- lethal time
- time of application
- Nezara viridula
How to Cite
Raharjo, L. A., Suryaminarsih, P., Mujoko, T., Rahmadhini, N., & Kusuma, R. M. (2022). Efficacy of Biopesticide Formula Containing Streptomyces sp. and Trichoderma sp. against Southern Green Stink Bug (Nezara viridula) on Soybean (Glycine max L.). Asian Research Journal of Agriculture, 15(4), 218-226. https://doi.org/10.9734/arja/2022/v15i4372
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Available:https://jatim.bps.go.id/statictable/2018/10/31/1342/wide-panen-productivity-dan-hasil-kedelai-di -jawa-timur-2002-2017.html
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Sidabutar M, Nuraida N, Sofian A. Pathogenicity of Trichoderma viride Fungus against Horn Beetle Larvae Pests on Oil Palm Plants. Journal of Agrofolium., 2022;2(2):135-141.
Safri M, Harijani WS, Suryaminarsih P. Pupa Viability Test of Fruit Flies (Bactrocera sp.) Become Imago by Giving Streptomyces sp Biological Agent. Agrotechnology-PLUMULA Scientific Periodic. 2017;5(1).
Fitriana IN, Suryaminarsih P, Mujoko T. Potential of Multientomopa Streptomyces sp. and Trichoderma sp. in Potato Extract Broth and Glucose Nitrate Broth Media on Pests (Spodoptera litura) Eating Behavior by in Vitro Test. Nusantara Science and Technology Proceedings. 2018:270-276.
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Mayaserli DP, Renowati R.. Utilization of Coconut Water as a Growth Media for Pseudomonas fluorescens and its Application as a Liquid Plant Fertilizer. Pioneer Health Journal. 2015;2(2):19-22.
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Bowling CC. The stylet sheath as an indicator of feeding activity by the southern green stink bug on soybeans. Journal of Economic Entomology. 1980;73(1):1-3
Trisnawati, Didin Julia, Wiwik Sri Harijani, and Penta Suryaminarsih. Concentration Test of Streptomyces sp. Biological Agent. against the Pupae of the Bactrocera sp Fruitfly. 2018;6(1):41–48.
Rawda M. Badawy and Hadeer I. Mohamed. Chitin extration, Composition of Different Six Insect Species and Their Comparable Characteristics with That of the Shrimp. J Am Sci 2015;11(6):127-134.
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Djojosumarto P. Complete guide to pesticides & their application. Agromedia; 2008.
Fitriana IN, Suryaminarsih P, Mujoko T. Potential of Multientomopa Streptomyces sp. and Tripchoderma sp. in Potato Extract Broth and Glucose Nitrate Broth Media on Pests (Spodoptera litura) Eating Behavior by in Vitro Test. Nusantara Science and Technology Proceedings. 2018:270-276.
Lu H, Rajamohan F, Dean DH. Identification of amino acid residues of Bacillus thuringiensis delta-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. Journal of Bacteriology. 1994;176(17):5554-5559.
Handayani FF, Sentat T, Rahim A. Acute Toxicity Test of Ethanol Extract of Selutui Puka Leaves (Tabernae montana macrocarpa Jack.) on (Artemia salina Leach) larvae. Journal of the World of Pharmacy. 2019;4(1):1-7.
Hasyim A, Lukman L, Marhaeni LS. Evaluation of lethal concentration and lethal time of botanical insecticides on onion caterpillar (Spodoptera exigua) in the laboratory. Journal of Horticulture. 2019;29(1):69-80.
Niâ L, Himawan T, Mudjiono G. Pathogenicity test of entomopathogenic fungus Metarhizium anisopliae (Moniliales: Moniliaceae) against Lepidiota stigma F. (Coleoptera: Scarabaeidae). Journal of Plant Pests and Diseases. 2016;4(1):24-31.
Cahyani NDKS, Wiadnya IBR, Khusuma A, Getas IW. Analysis of Lethal Concentration and Lethal Time of Aspergillus Niger Fungus Isolate Against Aedes Aegypti. Journal of Bioscience Medical Analysts (JAMBS). 2022;9(2): 78-86.
Available:https://jatim.bps.go.id/statictable/2018/10/31/1342/wide-panen-productivity-dan-hasil-kedelai-di -jawa-timur-2002-2017.html
Accessed on July 3, 2021.
Radiyanto B, Sodiq M, Nurcahyani NM. Diversity of insect pests and natural enemies on soybean plantations in Balong-Ponorogo District. Indonesian Journal of Entomology. 2010;7(2):116-116.
Hasibuan SA, Zurni TC, Syamsuddin S. The Effectiveness of BioPriming Trichoderma harzianum and Compost Application of Oil Palm Empty Fruit Bunches on Growth and Production of Soybean (Glycine max L. Merr.) Plants. Journal of Agrista. 2022;26(1):9-16.
Saputri E, Lisnawita L, Pinem MI. Encapsulation of Several Types of Trichoderma sp. on Soybean Seeds to Control Disease Sclerotium rolfsii Sacc. Journal of Agroecotechnology, University of North Sumatra. 2015;3(3):105478.
Ritonga NF, Nuraida N, Sari A. Pathogenicity of Trichoderma harzianum to Larval Pests of Horn Beetle (Oryctes rhinoceros) in Oil Palm Plants (Elaeis guineensis Jacq.) in the Laboratory. Journal of Agrofolium. 2022;2(2):98-107.
Hidayah AR, Harijani WS, Widajati W, Ernawati D. Potential entomo pathogenic fungi Metarhizium anisopliae, Beauveria bassiana and Streptomyces sp. on mortality of Lepidiota stigma in sugarcane. Plumula: Agrotechnology Scientific Periodic. 2019;7(2):64-72.
Vurukonda SSKP, Giovanardi D, Stefani E. Plant growth promoting and biocontrol activity of Streptomyces spp. as endophytes. International Journal of Molecular Sciences.2018;19(4):952.
Sidabutar M, Nuraida N, Sofian A. Pathogenicity of Trichoderma viride Fungus against Horn Beetle Larvae Pests on Oil Palm Plants. Journal of Agrofolium., 2022;2(2):135-141.
Safri M, Harijani WS, Suryaminarsih P. Pupa Viability Test of Fruit Flies (Bactrocera sp.) Become Imago by Giving Streptomyces sp Biological Agent. Agrotechnology-PLUMULA Scientific Periodic. 2017;5(1).
Fitriana IN, Suryaminarsih P, Mujoko T. Potential of Multientomopa Streptomyces sp. and Trichoderma sp. in Potato Extract Broth and Glucose Nitrate Broth Media on Pests (Spodoptera litura) Eating Behavior by in Vitro Test. Nusantara Science and Technology Proceedings. 2018:270-276.
Avrianto NI. The Effect of Application of the Biological Agent Formula of Streptomyces sp. and Trichoderma sp. On the Presence of Insect Pests on Soybean Plants (Glycines Max L. Merril) Vegetative Phase (Doctoral dissertation, "Veteran" National Development University, East Java); 2021.
Mayaserli DP, Renowati R.. Utilization of Coconut Water as a Growth Media for Pseudomonas fluorescens and its Application as a Liquid Plant Fertilizer. Pioneer Health Journal. 2015;2(2):19-22.
Breza Boruta B, Paluszak Z. The antagonistic activity of actinomycetes of Streptomyces genus in relation to Trichoderma koningii. Journal of Ecological Engineering. 2016;17(1):106-113.
Sinclair JB, Dhingra OD. Basic plant pathology methods. CRC press; 2017.
Bowling CC. The stylet sheath as an indicator of feeding activity by the southern green stink bug on soybeans. Journal of Economic Entomology. 1980;73(1):1-3
Trisnawati, Didin Julia, Wiwik Sri Harijani, and Penta Suryaminarsih. Concentration Test of Streptomyces sp. Biological Agent. against the Pupae of the Bactrocera sp Fruitfly. 2018;6(1):41–48.
Rawda M. Badawy and Hadeer I. Mohamed. Chitin extration, Composition of Different Six Insect Species and Their Comparable Characteristics with That of the Shrimp. J Am Sci 2015;11(6):127-134.
Sowmya B, Gomathi D, Kalaiselvi M, Ravikumar G, Arulraj C, Uma C. Production and Purification of Chitinase by Streptomyces sp. from Soil. Journal of Advanced Scientific Research. 2012; 3(3).
Djojosumarto P. Complete guide to pesticides & their application. Agromedia; 2008.
Fitriana IN, Suryaminarsih P, Mujoko T. Potential of Multientomopa Streptomyces sp. and Tripchoderma sp. in Potato Extract Broth and Glucose Nitrate Broth Media on Pests (Spodoptera litura) Eating Behavior by in Vitro Test. Nusantara Science and Technology Proceedings. 2018:270-276.
Lu H, Rajamohan F, Dean DH. Identification of amino acid residues of Bacillus thuringiensis delta-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. Journal of Bacteriology. 1994;176(17):5554-5559.
Handayani FF, Sentat T, Rahim A. Acute Toxicity Test of Ethanol Extract of Selutui Puka Leaves (Tabernae montana macrocarpa Jack.) on (Artemia salina Leach) larvae. Journal of the World of Pharmacy. 2019;4(1):1-7.
Hasyim A, Lukman L, Marhaeni LS. Evaluation of lethal concentration and lethal time of botanical insecticides on onion caterpillar (Spodoptera exigua) in the laboratory. Journal of Horticulture. 2019;29(1):69-80.
Niâ L, Himawan T, Mudjiono G. Pathogenicity test of entomopathogenic fungus Metarhizium anisopliae (Moniliales: Moniliaceae) against Lepidiota stigma F. (Coleoptera: Scarabaeidae). Journal of Plant Pests and Diseases. 2016;4(1):24-31.
Cahyani NDKS, Wiadnya IBR, Khusuma A, Getas IW. Analysis of Lethal Concentration and Lethal Time of Aspergillus Niger Fungus Isolate Against Aedes Aegypti. Journal of Bioscience Medical Analysts (JAMBS). 2022;9(2): 78-86.
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