Callus Induction from Zygotic Embryos of Coconut MATAG F2
A. R. Zuraida *
Biotechnology and Nanotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
G. Sentoor Kumaran
Crop Science and Soil Science Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
N. Ahmad
Crop Science and Soil Science Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
M. S. Syahirah Farhanah
Biotechnology and Nanotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
O. Ayu Nazreena
Biotechnology and Nanotechnology Research Centre, MARDI Headquarters, Persiaran MARDI-UPM, 43400 Serdang Selangor, Malaysia
*Author to whom correspondence should be addressed.
Abstract
The effect of 2,4-D (in concentrations of 5.0, 10.0, 20.0 and 40.0 mg/L) applied in combination with BAP (1.0, 10.0 and 20.0 mg/L) on callus induction in coconut MATAG F2 zygotic embryos cultured on Murashige and Skoog (MS) medium was investigated. The effects of IBA, TDZ and NAA combined with 2,4-D were also tested. The best callus formation (20%) was obtained on MS medium supplemented with 2,4-D at 10.0 mg/L. The induced calli were yellowish in colour and structurally compact. Different portions of the zygotic embryo were also compared for callus induction when used as explants and cultured on MS medium supplemented 10.0 mg/L 2,4-D. The incidence of callus formation (up to 83%) was highest from the middle portion of the embryos.
Keywords: 2,4-D, plant growth regulator, MATAG coconut, zygotic embryo, callus induction