Total Phenolic Compounds Extraction in Leaves of Ocimum gratissimum L. and Their Potential Activity against Some Agricultural Contaminants
Asian Research Journal of Agriculture,
Aims: Phenolic compounds are secondary metabolites that are important in the plant due to their role in plant defense and their antioxidant activity with other biological properties such as antipyretic, analgesic and antimicrobial activities. This study focused on the biological potential activity of total phenolic compounds extracted by soxhlet method from Ocimum gratissimum leaves (Lamiaceae), a popular medicinal plant harvested at Daloa (Côte d’Ivoire).
Study Design: Activities were directed on the in vitro antifungal and antioxidant activities combined to phenolic compounds analysis.
Place and Duration of Study: The study was carried out at the Department of Environment and Plant Protection, and Laboratory of Chemistry and Biochemistry, University of Agricultural Sciences and Veterinary Medicine (USAMV) between March to July 2014, Laboratory of Biochemistry and Microbiology (Bioactives Natural Substances Unit), Jean Lorougnon Guédé University between September 2014 to January 2015.
Methodology: The extract obtained named TPCOG was tested against Fusarium species for its antifungal activity by applying agar slant double dilution method and for its antioxidant activity by DPPH radical scavenging assay. Qualitative and quantitative evaluation of phenolic compounds were carried out by HPLC analysis method with sigma chemical standards.
Results: Tests showed that TPCOG was a powerful antifungal extract with MIC and MFC ranging between 3.125 µg/mL to 12.5 µg/mL. This extract was fungicidal and its antioxidant activity reached F= 541.25± .25 mM Trolox/mL (I= 70 ±1.85%) with a total phenolic content equal 195.70±1.33 mg GAE/g. Radical scavenge and antifungal activities correlated very well with total phenolic compounds. Phenolic content analysis showed presence of phenolic acids and flavonoids with a higher concentration for Quercetin (393.475 mg/100 g sample).
Conclusion: All this results demonstrated the biological potential activity of total phenolic compounds extracted from leaves of O. gratissimum and the possibility to use it in replace to essential oil for the formulation of biofungicides and nutraceuticals by industries.
- phenolic compounds
- Ocimum gratissimum
How to Cite
Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahu A. Indian medicinal herbs as sources of antioxidants. Food Research International. 2008;41:1- 15.
Omodamiro OD and Jimoh. MA. Antioxidant and Antibacterial activities of Ocimum gratissimum. American Journal of Phytomedicine and Clinical Therapeutics. 2015;3(01):010-019.
Tarkang PA, Okalebo FA, Siminyu JD, Ngugi WN, Mwaura AM, Mugweru J, Agbor GA, Guantai AN. Pharmacological evidence for the folk use of Nefang: Antipyretic, anti-inflammatory and antinociceptive activities of its constituent plants. BMC Complement. Altern. Med. 2015;15:174.
Ajayi AM, Tanayen JK, Ezeonwumelu J, Dare S, Okwanachi A, Adzu B, Ademowo OG. Anti-inflammatory, anti-nociceptive and total polyphenolic content of hydroethanolic extract of Ocimum gratissimum L. leaves. Afr. J. Med. Med. Sci. 2014;43:215–224.
Elisée KK, Sitapha O, Cendrine S, Sylvie F Benoit F. Etude de quelques propriétés biologiques de Ocimum gratissimum L., une Lamiaceae récoltée à Daloa (Côte d'Ivoire). European Scientific Journal. 2018;14(3):477-493
Prabhu KS, Lobo R, Shirwaikar AA, Shirwaikar A. Ocimum gratissimum: a review of its chemical, pharmacological and ethnomedicinal properties, the Open Complementary Medicine Journal. 2009;1:1-15.
Bottalico A. Fusarium diseases of cereals: Species complex and related mycotoxin profiles, in Europe" Journal of Plant Pathology. 1998;80(2):85-103.
Bilgrami KS, Choudhary AK. Mycotoxins in preharvest contamination of agricultural crops In Eds. Sinha KK, Bhatnagar D, Mycotoxins in agriculture and food safety. Marcel Dekker, New York; 1998.
Bindu S, Padma K. In vitro antifungal potency of some plant extracts against Fusarium oxysporum. International Journal of Green Pharmacy. 2009;63-65.
Kporou KE, Coulibaly I, Rodica P, Pintea A, Ouattara S, Odagiu A. HPLC phenolic compounds analysis and antifungal activity of extracts from Cymbopogon citratus (DC) Stapf against Fusarium graminearum and Fusarium oxysporum sp tulipae. Journal of Scientific Research & Reports. 2017;15(1):1-11.
Alam S, Akhter N, Begum F, Banu MS, Islam MR, Chowdhary AN. Antifungal activities (in vitro) of some plant extracts and smoke on four fungal pathogens of different hosts. Pak J Biol Sci. 2002; 5:307-309.
Hibar K, Daami-Remadi M, El Mahjoub M. Effets de certains fongicides de synthèse et biologiques sur la croissance mycelienne et l’agressivite de Fusarium oxysporum sp. radicis-lycopersici. Tropicultura. 2007;25(3): 146-152.
Clarkson PM., Thompson HS. Antioxidants: What role do they play in physical activity and health? American Journal of Clinical and Nutrition. 2000;72:637S-646S.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry. 2006;97:654-660.
Kaur C, John FL, Brett AS. The Fusarium laboratory manual. Blackwell Publishing Professional. 1st Ed., Iowa, USA. 2006;215 pages.
Liyana-Pathirana Chandrika M. and Fereidoon Shahidi. Importance of Insoluble-Bound Phenolics to Antioxidant Properties of Wheat. Journa of Agriculture Food and Chemistry. 2006;54:1256-1264.
Naczk M, Shahidi F. Extraction and analysis of phenolics in food. Journal of Chromatography Analysis. 2004;1054:95-111.
Bonta KR. Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables. J Pharm Anal. 2017;7(6):349–64.
Tsimidou M, Papadopolus G, Boskou D. Phenolic compounds and stability of virgin olive oil part 1. Food Chemistry. 1992;45: 141–144.
Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology. 1995;28:25-30.
Lee SE, Hwang HJO and Ha JS. Screening of medicinal plant extracts for Antioxidant activity. Life Sci. 2003;73:167-179.
Abirami M, Muthuswamy S. Antioxidant potential, total phenolic and total flavonoids content of various extracts from whole plant of Polycarpaea corymbosa lam. Asian J Pharm Clin Res. 2013;6(4):121-4.
Stalikas CD. Phenolic acids and flavonoids: Occurrence and analytical methods. In Uppu RM, et al. Free radicals and antioxidant Protocols, Springer Science+Business Media LLC. 2010;65-90.
Shukla S, Mehta A, Bajpai VK, Shukla S. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem Toxicol. 2009; 47(9):2338-43.
Armitage P. Statistical Methods in Medicinal Research. Oxford: Blackwell Scientific. 1971; 200 pages.
Celso VN, Tania UN, Erika B, Abrahão FNM., Díogenes AGC. & Benedito PDF. Antibacterial activity of Ocimum gratissimum L. Essential Oil, Mem. Inst. Oswaldo Cruz. 1999;94 (5):675-678.
Neelima R, Deepak K, Hari OS, Ganesh P, Choubey SK. Qualitative and quantitative evaluation of secondary metabolites in leaves, roots, and stem of Cleome viscosa L. International Journal of Green Pharmacy. 2018;56-65
Shi. J, Yu J, Pohorly J, Young C, Bryan M, Wu Y. Optimization of the extraction of polyphenols from grapes seed meal by aqueous ethanol solution. Food Agric Environ. 2006;1:42–47.
Gabor M, Johan L. Electron transfer indoles, phenols and sulphite (SO32-) to chloride dioxide (ClO2), the Journal of Physical Chemistry. 1988;92(1):134-137.
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