Plant Regeneration from Mature Inflorescence of Date Palm by Using Moringa Extract
Asian Research Journal of Agriculture,
Plant tissue culture is becoming an important technique for the massive propagation of date palm and the available source lead to the success of this process. The mature female inflorescence is a novel and available source for in vitro propagation of date palm, nowadays become needed at the large scale to usage of organic nature materials in agriculture practice which are rich in plant growth regulators as well as micro elements. This study describes in vitro plant regeneration from mature female inflorescence explants of evaluating date palm (Phoenix dactylifera L.) by using the methanol extract of Moringa oleifera leaves which has great potential to be used as a natural source of nutraceutical in micropropagation. Mature inflorescences explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of an extract of Moringa leaves with or without add TDZ at 0.2 and 0.6 BA mg/l. Indoles, phenols, proteins, amino acids, and carbohydrates were determined. The addition of different concentrations of an extract of Moringa oleifera leaves with TDZ at 0.2 and 0.6 BA mg/l gave the highest significant average percentage of callus formation, friable callus, germination of embryos and numbers of embryos comparing with other treatments. Also, given the highest significant values of free amino acids and carbohydrates were noted in callus and embryo germination stage.
- Date palm
- mature female inflorescences
- In vitro
- moringa leaves.
How to Cite
Wikipedia. Date Palm; 2017.
Othmani A, Bayoudh C, Drira N, Trifi M. In vitro cloning of date palm Phoenix dactylifera L. cv. Deglet Bey by using embryogenic suspension and temporary immersion bioreactor (TIB). Biotechnol. &Biotechnol. EQ. 2009;23:1181-1185.
Mustafa NS, Taha RA, Hassan SAM, Ziad NSM, Mustafa EA. Overcoming phenolic accumulation of date palm in vitro culture using α-tochopherol and cold pre-treatment. Middle-East Journal of Scientific Research. 2013;15 (3):344-350.
Jain SM. Date palm biotechnology: Current status and prospective - An overview. Emir. J.Food Agric. 2012;24(5): 386-399.
Fki L, Masmoudi R, Drira N, Rival A. An optimised protocol for plant regeneration from embryogenic suspension cultures of date palm (Phoenix dactylifera L.) cv. Deglet Nour. Plant Cell Rep. 2003;21:517–524.
Abahmane L. Recent achievements in date palm (Phoenix dactylifera L.) tissues micropropagation from inflorescence. Emir. J. Food Agric. 2013;25(11):863-874.
Abul-Soad AA. Micropropagation of Date Palm Using Inflorescence Explants. Springer Science+Business Media B.V. Chapter. 2011;6:91.
Jennifer KC, Reflini Harry EI, Brian PF, Stephen PC, Peter DS. Effects of Picloram in Inflorescence Culture of Oil Palm. Sumatra Bioscience, Singapore, Johansen, D.A. 1940. Plant Microtechnique. McGraw. 2010;71–78.
Feher A. Why somatic plant cells start to form embryos? In: Plant cell monographs. Mujib A., Samaj J. (eds), Springer-Verlag Berlin Heidelberg. 2006;2:85-101.
Lu C-Y. The use thidiazuron in tissue culture. In Vitro Cell. Dev. Biol. 1993;29: 92-96.
Passey A, Barrett K, James D. Adventitious shoot regeneration from seven commercial strawberry cultivars (Fragaria × ananassa Duch.) using a range of explant types. Plant Cell Rep. 2003; 21;397–401.
Murthy BN, Murch SJ, Saxena PK. Thidiazuron: A potent regulator of in vitro plant regeneration. In Vitro Cell. Dev. Biol. Plant. 1998;34;267–275.
Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A food plant with multiple medicinal uses. Phytother. Res. 2007;21: 17-25.
Marcu MG. Miracle tree. KOS Health Publications, Canada. 2005;108-115.
Fuglie LJ. The Miracle Tree: Moringa oleifera: Natural Nutrition for the Tropics. Church World Service, Dakar. 1999;68.
Basra SMA, Zahar M, Rehman H, Yasmin A, Munir H. Evaluating the response of sorghum and moringa (Moringa oleifera) leaf water extracts on seedling growth in hybrid maize applied through root media. In: Proceedings of the International Conference on Sustainable Food Grain production: Challenges and Opportunities. University of Agriculture, Faisalabad, Pakistan. 2009a;23.
Basra SMA, Zahoor R, Rehman H, Afzal I, Farooq M. Response of root applied brassica and moringa leaf water extracts on seedling growth in sunflower. In: Proceedings of the International Conference on Sustainable Food Grain Production: Challenges and Opportunities. University of Agriculture Faisalabad, Pakistan. 2009b;24
Noctor G, Foyer CH. Ascorbate and glutathione: Keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biololgy. 1998;49:249–279.
Foidl, N, Makkar HPS, Becker K. The potential of Moringa oleifera for agricultural and industrial uses. In: Fuglie, L.J. (eds.), The Miracle Tree: The Multiple Attributes of Moringa. Wageningen, The Netherlands. 2001;45–76.
El Award A. Moringa Tree: Nature’s Phamarcy.
Nagar PK, Leyer RI, Sircar PK. Cytokinins in developing fruits of Moringa pterigosperma Gaertn. Physiol. Plant. 2006;55:45-50.
Jacob SJP, Shenbagaraman S. Evaluation of antioxidant and antimicrobial activities of the selected green leafy vegetables. Int. J. Pharm. Tech. Res. 2011;3(1):148-152 .
Chance M, Maehly AC. Assay of catalases and peroxidases. Methods Enzymol. 1955; 2:764.
Giannopolitis CN, Ries SK. Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol. 1977l59:309-314.
Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin- Ciocalteu reagent. Nature Protocols. 2007; 2(4):875-877.
Yin H, Chen Q, Yi M. Effects of short term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum. Plant Growth Regul. 2008; 54:45-54.
Jackson ML. Soil Chemical Analysis. N.J Englewood Cliffs. Prentice Hall Inc. 1962; 151-185.
Bingham FT. Boron. In Methods of soil analysis. Am. Soc. Agron., Madison, WI, USA. 1982;431–447.
Fuglie L. New Uses of Moringa Studied in Nicaragua: ECHO's Technical Network Site-networking global hunger solutions: ECHO, Nicaragua; 2000.
Murashige T, Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 1962;15: 473-497.
Pottino BG. Methods in plant tissue culture. Dept. of Hort.,Agric.College.Maryland. Univ.,College Park.Maryland USA. 1981;8-29.
Larsen P, Harbo A, Klungroun S, Ashein T. On the biogenesis of some indole compounds in Acetobacter xylinum. Physiol Plant. 1962;15:552–565.
AOAC. Official Methods of Analysis. 13th Ed. Association of Official Analytical Chemists, Washington D.C. USA; 1995.
Bradford MM. A Rapid and Sensitive Method for the Quantitation of microgram quantities of protein utilizing the principal of protein – Dye Binding. Analytical Biochemistry. 1976;72:248-254.
Moore S, Stein WH. A modified ninhydrin reagent for the photometric determination of amino acids and related compounds. J. Biol. Chem.1954;211:907-913.
Dubois M, Smith F, Gilles KA, Hamilton JK, Rebers PA. Colorimetric method for determination of sugars and related substances. Annal. Chem. 1956;28(3):350-356.
Snedecor GW, Cochran WG. Statistical Methods. Seventh Edition, Iowa State Univ., Press Ames., Iowa, U.S.A; 1990.
Raj DE, Adri V, Cathleen M, Steven HS, Alexander AM. Optimization of a plant regeneration and genetic transformation protocol for Eucalyptus clonal genotypes. IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration and Delivery Arraial d’Ajuda, Bahia, Brazil; 2011.
Elahe B, Mohammad PP, Shahbazi E, Hosseini SZ. Effects of some plant growth regulators and light on callus induction and explants browning in date palm (Phoenix dactylifera L.) in vitro leaves culture. Iranian Journal of Plant Physiology. 2015; 5(4):1473-1481.
Hartinie M, Siti NSA, Nur SB, Jualang AG. In vitro Embryo Germination and Callus Induction of Cynometra cauliflora, an Underutilized Medicinal Plant. Transactions on Science and Technology. 2016;3(3):476-482.
Roberto C, Silvia S, Bruno M. The use of TDZ for the efficient in vitro regeneration andorganogenesis of strawberry and blueberry cultivars. Scientia Horticulturae. 2016;207:117–124.
Saifullah K, Tabassum BI. Direct shoot regeneration system for date palm (Phoenix dactylifera L.) cv. Dhakki as a means of micropropagation. Pak. J. Bot. 2012;44(6):1965-1971.
Zayed EMM. Propagation of Phoenix dactylifera L. Chamaerops humilis L. and Hyophorbe verschaffeltii L. palms by using tissue culture technique. Ph.D. Thesis, Department of Horticulture, Faculty of Agriculture, Cairo University, Egypt. 2011; 130–136.
Zayed Eman MM, Ola Abd Elbar H. Morphogenesis of immature female inflorescences of date palm in vitro. Annals of Agricultural Science. 2015;60(1):113–120.
Victor JMR, Murch SJ, Krishnaraj S, Saxena PK. Somatic embryogenesis and organogenesis in peanut: the role of thidizuron and N-benzylamino purine in the induction of plant morphogenesis. Plant Growth Regul. 1999;28:9- 15.
Sidky RA, Zaid ZE. Direct production of somatic embryos and plant regeneration using TDZ and CPPU of date palm (Phoenix dactylifera L.). International Journal of Academic Research. 2011;3(2): 792.
Madhavan J, Bollarapu S, Nandiganti MM, Pranab KM. In vitro somatic embryogenesis and plantlet regeneration from immature male inflorescence of adult dura and tenera palms of Elaeis guineensis (Jacq.). Springer. web page. 2015;4:256.
Abdulminam H, Almusawi A, Ansam S, Alshnawa M, Aqeel A, Alkhalifa S, Rayad FTA. Induction of direct somatic embryogenesis in date palm (Phoenix dactylifera L.) cv. Hellawi. Advances in Agriculture & Botanics- International Journal of the Bioflux Society. 2015;7(2): 80-89.
Jayanthi M, Murali Mohan N, Mandal P. Direct somatic embryogenesis and plantlet regeneration in oil palm. Journal of Plant Biochemistry and Biotechnology. 2011; 20:249-25.
Amal El-Ashry AE, Ahmed MMG, Shawky Bekheet AE. Zeatin and Thidiazuron Induced Embryogenic Calli From In Vitro Leaf and Stem of Jojoba (Simmondsia chinensis). Pakistan Journal of Biological Sciences. 2017;20:355-364.
Huetteman A, Preece EJ. Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell. Tiss. Org. Cult. 1993l33:105-119.
Satulyyra, Amparolima, Scott AM. In vitro regeneration of Salix nigra from adventitious shoots. Tree Physiology, 2006;26:969-975.
Zahoor AS, Faheem A. Effect of thidiazuron (TDZ) on in vitro micropropagation of Solanum tuberosum cvs. Desiree and Cardinal. Pak. J. Bot. 2009;41(4):1811-1815.
Mamaghani BA, Ghorbanli M, Assarehand MH, Zare AG. In vitro propagation of three Damask Roses accessions'.Iranian Journal of Plant Physiology. 2010;1:85-94.
Jia-Hua F, Jen-Tsung C. A Novel In Vitro Protocol for Inducing Direct Somatic Embryogenesis in Phalaenopsis aphrodite without Taking Explants. The Scientific World Journal. web page; 2014.
Guo B, Abbasi BH, Zeb A, LL Xu, Wei YH. Thidiazuron: A multi-dimensional plant growth regulator. African Journal of Biotechnology. 2011;10:8984–9000.
Allan MS. Structure and Function of Plant Cell Wall Proteins. The Plant Cell, American Society of Plant Physiologists. 1993;5:9-23.
Felix H, Daniel Koshland E. Protein Biochemistry. Encyclopedia Britannica, web: page; 2017.
Swamy MK, Mohanty SK, Anuradha M. The effect of plant growth regulators and natural supplements on in vitro propagation of Pogostemon cablin Benth. Journal of Crop Science Biotechnology. 2014;17(2):71-78.
Rasmia S, Darwesh S, Amal FM, Zein El-Din, Eman Zayed MM, Gehan Salama MY. Inducing Date Palm Somatic Embryogenesis and Plantlets Growth Using Date Palm Pollen Grains Extract. Egypt. J. Hort. 2015;42(1):391-405.
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