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In Bangladesh, the average yield of blackgram is not satisfactory level. There are many reasons for a lower yield of blackgram. Nitrogen and weed management in winter season is of them. This study is to find out the effect of nitrogen sources on the yield of different black gram varieties. The experiment was laid out in a split-plot Design with three replications. Nitrogen source was assigned to main plots and varieties to sub-plots. The field experiment was conducted at the Agronomy field of Central Research Farm of Sher-e-Bangla Agricultural University, Dhaka, Bangladesh during the period from September 2014 to January 2015. The experiment consisted of two factors such as nitrogen sources and blackgram varieties. The treatments were as follows: Factor A: nitrogen sources (3 types) viz. T1: prilled urea, T2: biofertilizer, T3: no nitrogen (control) and Factor B: varieties (4 types) viz. V1: BARI mash-1, V2: BARI mash-2, V3: BARI mash-3 and V4: local mash. In case of different nitrogen sources, the highest grain yield and harvest index (1.45 t ha-1 and 40.05%) was found from biofertilizer application. This treatment also showed highest pod length (5.28 cm), pods plant-1 (34.80), seeds pod-1 (7.08) and 1000-seed weight (40.55 g). Among the blackgram varieties, BARI Mash-3 gave the highest pod length (5.60 cm), pods plant-1 (39.93), seeds pod-1 (7.57) and 1000-seed weight (45.73 g), grain yield and harvest index (1.49 t ha-1 and 40.49%). These findings we observed that BARI Mash-3 applied with biofertilizer produced the highest grain yield and harvest index (1.84 t ha-1 and 48.04%).